latex beads Search Results


igg fitc conjugated latex bead phagocytosis assay kit  (Cayman Chemical)
94
Cayman Chemical igg fitc conjugated latex bead phagocytosis assay kit
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Igg Fitc Conjugated Latex Bead Phagocytosis Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/bio_rxiv__2020__04__25__061531-216-0-10?v=Cayman+Chemical
Average 94 stars, based on 1 article reviews
igg fitc conjugated latex bead phagocytosis assay kit - by Bioz Stars, 2026-08
94/100 stars
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latex beads  (Assay Designs Inc)
90
Assay Designs Inc latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Beads, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pmc03291473-76-16-21?v=Assay+Designs+Inc
Average 90 stars, based on 1 article reviews
latex beads - by Bioz Stars, 2026-08
90/100 stars
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redfluorescent latex beads  (Polysciences inc)
90
Polysciences inc redfluorescent latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Redfluorescent Latex Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/10__1002_slash_lno__11188-159-8-11?v=Polysciences+inc
Average 90 stars, based on 1 article reviews
redfluorescent latex beads - by Bioz Stars, 2026-08
90/100 stars
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0.5- m-diameter fluoresbrite yg fluorescent latex beads  (Polysciences inc)
90
Polysciences inc 0.5- m-diameter fluoresbrite yg fluorescent latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
0.5 M Diameter Fluoresbrite Yg Fluorescent Latex Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/10__1128_slash_aem__68__10__4772___4779__2002-89-5-11?v=Polysciences+inc
Average 90 stars, based on 1 article reviews
0.5- m-diameter fluoresbrite yg fluorescent latex beads - by Bioz Stars, 2026-08
90/100 stars
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fitc-conjugated beads cayman 500290  (Cayman Chemical)
90
Cayman Chemical fitc-conjugated beads cayman 500290
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fitc Conjugated Beads Cayman 500290, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pmc09701141-312-10-12?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
fitc-conjugated beads cayman 500290 - by Bioz Stars, 2026-08
90/100 stars
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fluorescence-labeled latex beads  (PolyScience)
90
PolyScience fluorescence-labeled latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fluorescence Labeled Latex Beads, supplied by PolyScience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/us07601363-121-0-5?v=PolyScience
Average 90 stars, based on 1 article reviews
fluorescence-labeled latex beads - by Bioz Stars, 2026-08
90/100 stars
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fluorescent latex beads  (Becton Dickinson)
90
Becton Dickinson fluorescent latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fluorescent Latex Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/10__3402_slash_polar__v34__23349-83-0-3?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
fluorescent latex beads - by Bioz Stars, 2026-08
90/100 stars
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3-µm latex nh2-beads  (PolyScience)
90
PolyScience 3-µm latex nh2-beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
3 µm Latex Nh2 Beads, supplied by PolyScience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pm36497137-63-5-7?v=PolyScience
Average 90 stars, based on 1 article reviews
3-µm latex nh2-beads - by Bioz Stars, 2026-08
90/100 stars
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fitc-conjugated polystyrene beads  (Polysciences inc)
90
Polysciences inc fitc-conjugated polystyrene beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fitc Conjugated Polystyrene Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pmc04138970-105-15-20?v=Polysciences+inc
Average 90 stars, based on 1 article reviews
fitc-conjugated polystyrene beads - by Bioz Stars, 2026-08
90/100 stars
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fitcfluorescent diameter latex beads  (Polysciences inc)
90
Polysciences inc fitcfluorescent diameter latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fitcfluorescent Diameter Latex Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pm22357942-151-34-40?v=Polysciences+inc
Average 90 stars, based on 1 article reviews
fitcfluorescent diameter latex beads - by Bioz Stars, 2026-08
90/100 stars
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fluorescent latex counting beads becton dickinson  (Becton Dickinson)
90
Becton Dickinson fluorescent latex counting beads becton dickinson
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fluorescent Latex Counting Beads Becton Dickinson, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pmc03698069-229-26-30?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
fluorescent latex counting beads becton dickinson - by Bioz Stars, 2026-08
90/100 stars
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nonfluorescent latex beads  (Polysciences inc)
90
Polysciences inc nonfluorescent latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Nonfluorescent Latex Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latex+beads/pmc03317272-91-13-16?v=Polysciences+inc
Average 90 stars, based on 1 article reviews
nonfluorescent latex beads - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .

Journal: bioRxiv

Article Title: Recombinant BCG overexpressing a STING agonist elicits trained immunity and improved antitumor efficacy in non-muscle invasive bladder cancer

doi: 10.1101/2020.04.25.061531

Figure Lengend Snippet: a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .

Article Snippet: IgG-FITC conjugated latex bead phagocytosis assay kit (Item No. 500290, Cayman Chemicals, USA) was used for phagocytosis studies.

Techniques: Flow Cytometry, Activity Assay, Labeling, Staining, Infection, Microscopy, Software, Fluorescence