latex beads Search Results


igg fitc conjugated latex bead phagocytosis assay kit  (Cayman Chemical)
94
Cayman Chemical igg fitc conjugated latex bead phagocytosis assay kit
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Igg Fitc Conjugated Latex Bead Phagocytosis Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igg fitc conjugated latex bead phagocytosis assay kit/product/Cayman Chemical
Average 94 stars, based on 1 article reviews
igg fitc conjugated latex bead phagocytosis assay kit - by Bioz Stars, 2026-03
94/100 stars
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carboxylic blue latex beads cab400nm  (Magsphere)
90
Magsphere carboxylic blue latex beads cab400nm
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Carboxylic Blue Latex Beads Cab400nm, supplied by Magsphere, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/carboxylic blue latex beads cab400nm/product/Magsphere
Average 90 stars, based on 1 article reviews
carboxylic blue latex beads cab400nm - by Bioz Stars, 2026-03
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latex beads (1:5,000)  (Polysciences inc)
90
Polysciences inc latex beads (1:5,000)
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Beads (1:5,000), supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/latex beads (1:5,000)/product/Polysciences inc
Average 90 stars, based on 1 article reviews
latex beads (1:5,000) - by Bioz Stars, 2026-03
90/100 stars
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latex beads  (Bangs Laboratories)
90
Bangs Laboratories latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Beads, supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/latex beads/product/Bangs Laboratories
Average 90 stars, based on 1 article reviews
latex beads - by Bioz Stars, 2026-03
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protein a polystyrene microspheres bangs laboratories  (Bangs Laboratories)
90
Bangs Laboratories protein a polystyrene microspheres bangs laboratories
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Protein A Polystyrene Microspheres Bangs Laboratories, supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein a polystyrene microspheres bangs laboratories/product/Bangs Laboratories
Average 90 stars, based on 1 article reviews
protein a polystyrene microspheres bangs laboratories - by Bioz Stars, 2026-03
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latex beads-rabbit igg-fitc  (Cayman Chemical)
90
Cayman Chemical latex beads-rabbit igg-fitc
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Beads Rabbit Igg Fitc, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/latex beads-rabbit igg-fitc/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
latex beads-rabbit igg-fitc - by Bioz Stars, 2026-03
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latex beads  (PolyScience)
90
PolyScience latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Beads, supplied by PolyScience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/latex beads/product/PolyScience
Average 90 stars, based on 1 article reviews
latex beads - by Bioz Stars, 2026-03
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fitcfluorescent diameter latex beads  (Polysciences inc)
90
Polysciences inc fitcfluorescent diameter latex beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fitcfluorescent Diameter Latex Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitcfluorescent diameter latex beads/product/Polysciences inc
Average 90 stars, based on 1 article reviews
fitcfluorescent diameter latex beads - by Bioz Stars, 2026-03
90/100 stars
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fluorescent latex counting beads becton dickinson  (Becton Dickinson)
90
Becton Dickinson fluorescent latex counting beads becton dickinson
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Fluorescent Latex Counting Beads Becton Dickinson, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent latex counting beads becton dickinson/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
fluorescent latex counting beads becton dickinson - by Bioz Stars, 2026-03
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latex (2- m diameter) beads  (Polysciences inc)
90
Polysciences inc latex (2- m diameter) beads
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex (2 M Diameter) Beads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/latex (2- m diameter) beads/product/Polysciences inc
Average 90 stars, based on 1 article reviews
latex (2- m diameter) beads - by Bioz Stars, 2026-03
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latex bead-containing phagosomes  (Blackwell Science Ltd)
90
Blackwell Science Ltd latex bead-containing phagosomes
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Bead Containing Phagosomes, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/latex bead-containing phagosomes/product/Blackwell Science Ltd
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latex bead-containing phagosomes - by Bioz Stars, 2026-03
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latex immunoassay liatest d-di  (Diagnostica Stago)
90
Diagnostica Stago latex immunoassay liatest d-di
a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of <t>FITC-labeled</t> <t>IgG-opsonized</t> latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .
Latex Immunoassay Liatest D Di, supplied by Diagnostica Stago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .

Journal: bioRxiv

Article Title: Recombinant BCG overexpressing a STING agonist elicits trained immunity and improved antitumor efficacy in non-muscle invasive bladder cancer

doi: 10.1101/2020.04.25.061531

Figure Lengend Snippet: a . Percentages of M1- and M2-macrophages and M-MDSCs arising from primary murine macrophages. b . Percentages of inflammatory, TNF-α+ M1, and IL-6 + M1 macrophages, and c . M2 and IL-10 + M2 macrophages arising from primary human macrophages. Data were collected after 24 hr exposures at MOI of 20:1 as determined by flow cytometry using gating schemes shown in Fig. S8-S13 . Data are SEM (n = 3 replicates). d . Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. e . Autophagy induction and f . quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h . Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was by mean fluorescence intensity. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by 2-tailed Student’s t-test. Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Fig. S9 and S11 .

Article Snippet: IgG-FITC conjugated latex bead phagocytosis assay kit (Item No. 500290, Cayman Chemicals, USA) was used for phagocytosis studies.

Techniques: Flow Cytometry, Activity Assay, Labeling, Staining, Infection, Microscopy, Software, Fluorescence